Ribes plants,like most of other fruit trees ,are characterized by their large number but small size of somatic chromosomes.In the light of these spccial characteristies,we have examined several factorial combinations of material,pretreatment,slide preparing method and stain to identify the most promising techique for studying the chromosomes and further analyzing the karyotypes and chromosome banding of Ribes plants.The resuts indicated that the combination of root tip with pretreatment of 2m molL^-1 8-hydroxyquinoline plus 0.05% colchicine or 0.3% balm for 3-5 hours at 14℃,pre-hypotonic treatment of 0.07mol L^-1 KCl for 30min,fixation in Carnoy"s fluid,hydrolysis in 5% cellulase and 5% pectinase mixture for 4-5 hours at 25℃,post-hypotoic treatment in distilled water for half an hour and staining in Giemsa Could make the chromosome preparation superior to other treatment combinations.【A Comparison of Techniques for Preparing Somatic Chromosomes in Ribes Species】完成機構:NortheastAgriculturalUniversity,Harbin150030,P.R.China
猜你喜歡
- 跪求 NVIDIA GeForce 9600GT 最新顯卡驅動
- force 在英語中是什么意思?
- Fort Minor
- for example與such as的區別 不同英語詞組的用法
- 電腦藍屏顯示:collecting data for crash dump 怎么處理啊,求高手幫忙
- NVIDIAQuadro P600與NVIDIA GeForce GTX 1050Ti哪個好
- uniform是什么意思中文 uniform的意思介紹
- 因此的英文短語有哪些? 因此的英文短語
- byford是什么品牌
- java.lang.NumberFormatException
